Discover Community Create You

Loading publication...

Noodle always counts identifier-free feature events for service operation. With your permission, Google Analytics can also estimate audience and engagement. Search text, publication identifiers, cookies, IP addresses and browser fingerprints are never included in Noodle's first-party event counters.

Privacy details

Article

Development of a protocol for multiple staining with fluorochromes to assess the functional status of boar spermatozoa

28 Nov 2005

Abstract excerpt

The aim of this study was to design a simple and reliable method for the simultaneous evaluation of the nucleus, the acrosome, and the mitochondrial sheath of boar spermatozoa. Sperm samples coming from healthy and sexually mature Pietrain boars were incubated with two nuclear fluorochromes--bis-benzamide specific for viable cells, and propidium iodide specific for nonviable cells--the fluorochrome Mitotracker...

Read the complete abstract on PubMed

Topics

Share this publication in a Topic to start or enrich a Post.

Share to Reproductive Biology and Fertility
Share to Reproductive biology and impacts on aquatic species
Share to Sperm and Testicular Function

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Source

Identifiers

PMID: 16315232
DOI: 10.1002/jemt.20246

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 8 Sept 2026.

Related research

Semantic proximity does not establish scientific evidence.

Research trail→

Click a neighbor to travelStep 1 · 12 closest

Development of a protocol for multiple staining with fluorochromes to assess the functional status of boar spermatozoaPMID 16315232Read ↗

Validation of a new multiparametric protocol to assess viability, acrosome integrity and mitochondrial activity in cooled and frozen thawed boar spermatozoaSemantic proximity Read ↗

Evaluation of the one-step eosin-nigrosin staining technique for human sperm vitality assessmentSemantic proximity Read ↗

The MTT assay application to measure the viability of spermatozoa: a variety of the assay protocolsSemantic proximity Read ↗

Assessment of Different Functional Parameters of Frozen–Thawed Buffalo Spermatozoa by Using Cytofluorimetric DeterminationsSemantic proximity Read ↗

Motility Assessment of Porcine Spermatozoa: a Comparison of MethodsSemantic proximity Read ↗

In Vitro Measures for Assessing Boar Semen FertilitySemantic proximity Read ↗

The combination of kinetic and flow cytometric semen parameters as a tool to predict fertility in cryopreserved bull semenSemantic proximity Read ↗

Simple histochemical stain for acrosomes on sperm from several speciesSemantic proximity Read ↗

A Flow Cytometric Method for Rapid Determination of Sperm Concentration and Viability in Mammalian and Avian SemenSemantic proximity Read ↗

Assessment of Different Sperm Quality Parameters to Predict <i>in vitro</i> Fertility of BullsSemantic proximity Read ↗

Assessment of the acrosomal status of membrane-intact ram spermatozoa after freezing and thawing, by simultaneous lectin/Hoechst 33258 stainingSemantic proximity Read ↗

Sperm function and oxidative status: Effect on fertility in Bos taurus and Bos indicus bulls when semen is used for fixed-time artificial inseminationSemantic proximity Read ↗

⌁Select a neighboring publication to make it the new centre.