Article
Efficient mutation detection in MEN1 gene using a combination of single-strand conformation polymorphism (MDGA) and heteroduplex analysis.
Electrophoresis - 1 Jan 2003
Crépin Michel, Escande Fabienne, Pigny Pascal, Buisine Marie-P, Calender Alain, Porchet Nicole, Odou Marie-Françoise
Abstract excerpt
For facilitated genotypic analysis of multiple endocrine neoplasia type 1 (MEN1), a familial syndrome associated with tumors of the parathyroid and neuroendocrine tissues, we developed two screening methods, heteroduplex mutation assay (HMA) and mutation detection gel analysis (MDGA), both based on electrophoretic discrimination of polymerase chain reaction (PCR) products, to detect the mutations. Forty-three...
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