Article
Mutation in a calpain-like protease affects the posttranslational mannosylation of phosphatases in Aspergillus nidulans.
Fungal genetics and biology : FG & B - 1 Mar 2003
Nozawa S R, May G S, Martinez-Rossi N M, Ferreira-Nozawa M S, Coutinho-Netto J, Maccheroni W, Rossi A
Abstract excerpt
In this communication, we show that the palB7 mutation drastically reduced the mannose and N-acetylgalactosamine content of the pacA-encoded acid phosphatase secreted by the fungus Aspergillus nidulans at pH 5.0, compared to a control strain. By using mRNA differential display reverse transcription and polymerase chain reaction, we isolated two cDNAs from the control pabaA1 strain that were not detected in the...
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