Article
[Effect of PCR reaction volume on the accuracy of human identification tests].
Fa yi xue za zhi - 1 Aug 2002
Zhou Huai-gu, Ping Yuan, Xu Qing-wen
Abstract excerpt
OBJECTIVE: To investigate the effect of different PCR amplification volume on the accuracy of human identification test. METHODS: Human genome DNA samples were amplified using ABI PRISM Profiler Plus kits in 50 microliters, 25 microliters, 12.5 microliters, and 6.25 microliters reaction volume, respectively. The thermocycle parameters were the same. All PCR products were then electrophoresized on ABI PRISM 310...
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