Article
[Molecular phylogeny of Giardia lamblia based on triose phosphate isomerase (tim) gene sequence].
Zhongguo ji sheng chong xue yu ji sheng chong bing za zhi = Chinese journal of parasitology & parasitic diseases - 1 Jan 2000
Li J H, Lu S Q, Zhang Y P, Wang F Y, Wen J F
Abstract excerpt
OBJECTIVE: To investigate the intraspecies phylogeny and genetic diversity of G. lamblia. METHODS: Total genomic DNA was extracted and partial fragments of tim gene were amplified by PCR. All the nucleotide sequences were analyzed by means of phylogenetic analysis, which were constructed with pasimony and N-J methods. RESULTS: A total of 124 variable sites (23% of the total sequences detected) were defined, most...
Topics
- Animals
- Base Sequence
- DNA, Protozoan
- Genetic Variation
- Giardia lamblia
- Molecular Sequence Data
- Phylogeny
- Polymerase Chain Reaction
- Sequence Analysis, DNA
- Triose-Phosphate Isomerase
