[Establishment of mouse ZF-12+/- embryonic stem cells].

Abstract excerpt

By using the mouse zinc finger protein gene ZF-12 genomic DNA fragment, pSSC-TV-10.5 was designed and constructed as a replacement vector. Structure of pSSC-TV-10.5 was identified by restrictive digestion analysis and partly sequencing. Then linearized vector was electroporated into ES cells, and transfected cells were screened by G418 and GANC selection. Among 508 G418r/GANCr colonies, 4 were proved to have...

Topics

Share this publication in a Topic to start or enrich a Post.

• Share to Blotting, Southern
• Share to Electroporation
• Share to Embryo, Mammalian