Article
Cloning and identification of a novel variant of human vascular endothelial growth factor.
Journal of Tongji Medical University = Tong ji yi ke da xue xue bao - 1 Jan 2001
Guo J, Qu S
Abstract excerpt
A novel variant of human vascular endothelial growth factor (h'VEGF165) cDNA was amplified by nested PCR method from the HL601 cells and was cloned into a eukaryotic expressing vector pcDNA3 to construct a recombinant plasmid pCD-h'VEGF165. The amplified h'VEGF165 cDNA fragment was identified by enzyme digestion and DNA sequencing methods. Also, wild-type hVEGF165 cDNA was obtained, identified and cloned into a...
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