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Effect of Cell Confluence on Production of Cloned Mice Using an Inbred Embryonic Stem Cell Line1

1 Feb 2003

Abstract excerpt

Mice have been successfully cloned from both somatic cells and hybrid embryonic stem (ES) cells. Heterozygosity of the donor ES cell genome has been suggested as a crucial factor for long-term survival of cloned mice. In the present study, an inbred ES cell line, HM-1 (129/Ola), and a well-tested ES cell line, R1 (129/Sv x 129/Sv-CP), were used as donor cells to evaluate the developmental potential of nuclear...

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PMID: 12533424
DOI: 10.1095/biolreprod.102.005819

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