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Article

Aromatic stacking as a determinant of the thermal stability of CYP119 from Sulfolobus solfataricus.

Archives of biochemistry and biophysics - 1 Jan 2003

Puchkaev Andrei V, Koo Laura S, Ortiz de Montellano Paul R

Abstract excerpt

Two notable features of the thermophilic CYP119, an Arg154-Glu212 salt bridge between the F-G loop and the I helix and an extended aromatic cluster, were studied to determine their contributions to the thermal stability of the enzyme. Site-specific mutants of the salt bridge (Arg154, Glu212) and aromatic cluster (Tyr2, Trp4, Trp231, Tyr250, Trp281) were expressed and purified. The substrate-binding and kinetic...

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PMID: 12464244
DOI: 10.1016/s0003-9861(02)00402-2

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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