Article
Strategies for PCR based detection of Burkholderia pseudomallei DNA in paraffin wax embedded tissues
28 Nov 2002
Abstract excerpt
Recently, several cases of melioidosis imported to Europe have been reported. The diagnosis of the acute or chronic infection remains challenging. This report describes an optimised protocol for fast and reliable DNA preparation for use in two different polymerase chain reaction (PCR) assays, namely: (1) a seminested PCR assay targeting a genus specific sequence of the ribosomal protein subunit 21 (rpsU) gene and...
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