Article
Real-time quantitative RT-PCR assay of GADD45 gene expression changes as a biomarker for radiation biodosimetry
1 Jan 2002
Abstract excerpt
PURPOSE: To assess the efficacy of fluorescent-based quantitative reverse transcription-polymerase chain reaction (QRT-PCR) technology to measure gene expression changes (GEC) for rapid, point-of-care radiation dose assessment. MATERIALS AND METHODS: A real-time QRT-PCR assay based on 5'-fluorogenic nuclease TaqMan(TM) methodology was developed, which employs both relative and absolute quantification of a...
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