Article
Web-based primer design for single nucleotide polymorphism analysis.
Trends in genetics : TIG - 1 Dec 2002
Neff Michael M, Turk Edward, Kalishman Michael
Abstract excerpt
The detection of single nucleotide polymorphisms by PCR is necessary for many types of genetic analysis, from mapping genomes to tracking specific mutations. This technique is most commonly used when polymorphisms alter restriction endonuclease recognition sites. Here we describe a web-based program, dCAPS Finder 2.0, that facilitates the design of mismatched PCR primers to create or remove a restriction...
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