Article
Selective genotyping of individual cells by capillary polymerase chain reaction.
Electrophoresis - 1 Sept 2002
Li Hanlin, Yeung Edward S
Abstract excerpt
On-line capillary polymerase chain reaction (PCR) coupled with laser-induced fluorescence detection was successfully demonstrated for individual human cells. A single 50 num inner diameter (ID) fused-silica capillary served both as the reaction vessel and for isolating single cells. SYBR Green I dye was added into the reaction mixture for dynamic fluorescence labeling. Because of the small ID of the capillary,...
Read the complete abstract on PubMedTopics
Share this publication in a Topic to start or enrich a Post.