Article
PCR‐based detection of<i>Pythium</i>and<i>Lagenidium</i>DNA in frozen and ethanol‐fixed animal tissues
1 Aug 2002
Abstract excerpt
Abstract The purpose of this study was to evaluate the application of previously described Pythium insidiosum‐ and Lagenidium ‐specific nested PCR assays to the detection of oomycete DNA in animal tissues. DNA was extracted from 15 frozen and 10 ethanol‐fixed tissues obtained from six animals with pythiosis, five animals with lagenidiosis, one animal with nonoomycotic skin disease and two animals without skin...
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