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Development of a Nested Polymerase Chain Reaction Assay for the Detection and Identification of <i>Pythium insidiosum</i>

1 Mar 2002

Abstract excerpt

Pythium insidiosum is an important cause of cutaneous and gastrointestinal disease in horses and dogs in the southeastern United States. Culture-based diagnosis of pythiosis is rarely definitive because production and identification of reproductive structures is difficult. The purpose of this study was to develop a polymerase chain reaction (PCR)-based assay for the identification of P insidiosum. Genomic DNA was...

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PMID: 11899029
DOI: 10.1111/j.1939-1676.2002.tb02346.x

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