Genotyping the mouse severe combined immunodeficiency mutation using the polymerase chain reaction with confronting two-pair primers (PCR-CTPP).

doi:10.1538/expanim.51.391

Article

Genotyping the mouse severe combined immunodeficiency mutation using the polymerase chain reaction with confronting two-pair primers (PCR-CTPP).

Experimental animals - 1 Jul 2002

Maruyama Chika, Suemizu Hiroshi, Tamamushi Shojiro, Kimoto Shigenobu, Tamaoki Norikazu, Ohnishi Yasuyuki

Abstract excerpt

An allele specific polymerase chain reaction with confronting two-pair primers (PCR-CTPP) was developed as an assay for genotyping the mouse Prkdcscid gene mutation (former name scid). The reverse primer (WR) was designed to include the antisense nucleotide (A) specific for the wild type allele at the 3' end with the counterpart forward primer (F) upstream. The other forward primer (MF) was designed to include...

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Genotyping the mouse severe combined immunodeficiency mutation using the polymerase chain reaction with confronting two-pair primers (PCR-CTPP).

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