Article
Genotyping of Francisella tularensis strains by pulsed-field gel electrophoresis, amplified fragment length polymorphism fingerprinting, and 16S rRNA gene sequencing.
Journal of clinical microbiology - 1 Aug 2002
García Del Blanco N, Dobson M E, Vela A I, De La Puente V A, Gutiérrez C B, Hadfield T L, Kuhnert P, Frey J, Domínguez L, Rodríguez Ferri E F
Abstract excerpt
We evaluated three molecular methods for identification of Francisella strains: pulsed-field gel electrophoresis (PFGE), amplified fragment length polymorphism (AFLP) analysis, and 16S rRNA gene sequencing. The analysis was performed with 54 Francisella tularensis subsp. holarctica, 5 F. tularensis subsp. tularensis, 2 F. tularensis subsp. novicida, and 1 F. philomiragia strains. On the basis of the combination...
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