Article
A cyclic AMP-dependent pathway regulates the expression of acetylcholinesterase during myogenic differentiation of C2C12 cells.
The Journal of biological chemistry - 27 Sept 2002
Siow Nina L, Choi Roy C Y, Cheng Anthony W M, Jiang Joy X S, Wan David C C, Zhu Shang Q, Tsim Karl W K
Abstract excerpt
The expression of acetylcholinesterase (AChE) is markedly increased during myogenic differentiation of C2C12 myoblasts to myotubes; the expression is mediated by intrinsic factor(s) during muscle differentiation. In order to analyze the molecular mechanisms regulating AChE expression during myogenic differentiation, a approximately 2.2-kb human AChE promoter tagged with a luciferase reporter gene, namely...
Topics
- Acetylcholinesterase
- Animals
- Binding Sites
- Blotting, Northern
- Cell Differentiation
- Cyclic AMP
- Cyclic AMP-Dependent Protein Kinases
- DNA
- DNA-Binding Proteins
- Genes, Reporter
- Humans
- Immunoblotting
- Luciferases