Article
Reactivity of recombinant and mutant vanadium bromoperoxidase from the red alga Corallina officinalis.
Journal of inorganic biochemistry - 25 Jul 2002
Carter Jayme N, Beatty Kimberly E, Simpson Matthew T, Butler Alison
Abstract excerpt
Vanadium bromoperoxidase (VBPO) from the marine red alga Corallina officinalis has been cloned and heterologously expressed in Esherichia coli. The sequence for the full-length cDNA of VBPO from C. officinalis is reported. Steady state kinetic analyses of monochlorodimedone bromination reveals the recombinant enzyme behaves similarly to native VBPO from the alga. The kinetic parameters (K(m)(Br-)=1.2 mM,...
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