Article
Cold-adaptation mechanism of mutant enzymes of 3-isopropylmalate dehydrogenase from Thermus thermophilus.
Protein engineering - 1 Jun 2002
Suzuki Toshiharu, Yasugi Masako, Arisaka Fumio, Oshima Tairo, Yamagishi Akihiko
Abstract excerpt
Random mutagenesis of Thermus thermophilus 3-isopropylmalate dehydrogenase revealed that a substitution of Val126Met in a hinge region caused a marked increase in specific activity, particularly at low temperatures, although the site is far from the binding residues for 3-isopropylmalate and NAD. To understand the molecular mechanism, residue 126 was substituted with one of eight other residues, Gly, Ala, Ser,...
Read the complete abstract on PubMedTopics
Share this publication in a Topic to start or enrich a Post.