Article
Analysis of mixed chimerism in patients after allogeneic stem cell transplantation using a capillary electrophoresis system.
Acta haematologica - 1 Jan 2002
Tsutsumi Y, Tanaka J, Kato N, Zhang L, Mori A, Kobayasi R, Kasai M, Asaka M, Imamura M
Abstract excerpt
We analyzed mixed chimerism (MC) after allogeneic stem cell transplantation (SCT) using a capillary electrophoresis system with four kinds of fluorescence-labeled primers for microsatellites (D3S1359, D6S89, ACTBP2, HGH). The sensitivities of all microsatellites were at least 3%. The present method is sufficiently rapid: only 3-4 h are needed to perform all the procedures. For analysis of MC in 30 patients who...
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