Article
Metabolic engineering of xanthophyll content in tomato fruits.
FEBS letters - 22 May 2002
Dharmapuri Sridhar, Rosati Carlo, Pallara Patrizia, Aquilani Riccardo, Bouvier Florence, Camara Bilal, Giuliano Giovanni
Abstract excerpt
Ripe tomato fruits accumulate significant amounts of the linear carotene lycopene, but only trace amounts of xanthophylls (oxygenated carotenoids). We overexpressed the lycopene beta-cyclase (b-Lcy) and beta-carotene hydroxylase (b-Chy) genes under the control of the fruit-specific Pds promoter. Transgene and protein expression was followed through semi-quantitative reverse transcription-PCR, Western blotting,...
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