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Article

Construction and application of epitope- and green fluorescent protein-tagging integration vectors for Bacillus subtilis.

Applied and environmental microbiology - 1 May 2002

Kaltwasser Marcus, Wiegert Thomas, Schumann Wolfgang

Abstract excerpt

Here we describe the construction and application of six new tagging vectors allowing the fusion of two different types of tagging sequences, epitope and localization tags, to any Bacillus subtilis protein. These vectors are based on the backbone of pMUTIN2 and replace the lacZ gene with tagging sequences. Fusion of the tagging sequences occurs by PCR amplification of the 3' terminal part of the gene of interest...

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Identifiers

PMID: 11976148
DOI: 10.1128/AEM.68.5.2624-2628.2002
PMCID: PMC127589

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 5 Sept 2026. Manifest publication-graph-cohort-2026-09-05.1.

Source receipt: pubmed, retrieved 5 Sept 2026.

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