Article
A series of double disruptants for protein phosphatase genes in Saccharomyces cerevisiae and their phenotypic analysis.
Yeast (Chichester, England) - 1 May 2002
Sakumoto Naoko, Matsuoka Itsumi, Mukai Yukio, Ogawa Nobuo, Kaneko Yoshinobu, Harashima Satoshi
Abstract excerpt
Thirty-two protein phosphatase (PPase) genes were identified in Saccharomyces cerevisiae based on the nucleotide sequences of the entire genome. In an effort to understand the role of PPases and their functional redundancy in the cellular physiology of one of the reference eukaryotic organisms, a series of single and double PPase gene disruptants were constructed in the W303 strain background. Two single...
Topics
- Calcium Chloride
- Carrier Proteins
- Cell Cycle Proteins
- Culture Media
- Dual-Specificity Phosphatases
- Fungal Proteins
- Gene Deletion
- Phenotype
- Phosphoprotein Phosphatases
- Protein Phosphatase 2
- Protein Phosphatase 2C
- Protein Tyrosine Phosphatases
- Saccharomyces cerevisiae