Article
Physical and genetic map of Enterococcus faecium ATCC19434 and demonstration of intra- and interspecific genomic diversity in enterococci.
FEMS microbiology letters - 5 Feb 2002
Oana Kozue, Okimura Yukie, Kawakami Yoshiyuki, Hayashida Nobuaki, Shimosaka Makoto, Okazaki Mitsuo, Hayashi Tetsuya, Ohnishi Makoto
Abstract excerpt
A physical map of the Enterococcus faecium ATCC19434 chromosome was constructed by NotI, I-CeuI and Sse8387I. The chromosome was a circular DNA of 2600 kb in size, and contained six rRNA operons (rrn). The locations and orientations of the six rrn operons and 24 different determinants were mapped. Genomes of three additional E. faecium strains were also analyzed by I-CeuI digestion, and the genome sizes were...
Topics
- Chromosomes, Bacterial
- Cloning, Molecular
- Deoxyribonucleases, Type II Site-Specific
- Endodeoxyribonucleases
- Enterococcus
- Enterococcus faecium
- Genetic Variation
- Genome, Bacterial
- Molecular Sequence Data
- Physical Chromosome Mapping
- rRNA Operon