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Dengue virus infection rate in field populations of female <i>Aedes aegypti</i> and <i>Aedes albopictus</i> in Singapore

1 Apr 2002

Abstract excerpt

We developed a single-step reverse transcription-polymerase chain reaction (RT-PCR) followed by a semi-nested PCR using an upstream consensus primer and four type-specific primers within the non-structural protein gene (NS3) of dengue viruses to type dengue viruses in field populations of female Aedes mosquitoes. This yielded diagnostic fragments of 169, 362, 265 and 426 base pairs for dengue virus types 1, 2, 3...

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PMID: 11952948
DOI: 10.1046/j.1365-3156.2002.00873.x

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