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Detection of Dengue Viruses in Field Caught Male Aedes aegypti and Aedes albopictus (Diptera: Culicidae) in Singapore by Type-Specific PCR

1 Jul 2001

Abstract excerpt

Field male Aedes aegypti (L.) and Aedes albopictus (Skuse) adults caught from fixed monitoring stations weekly for 1 yr were screened for dengue viruses (DEN-1, DEN-2, DEN-3, and DEN-4). The assay was carried out using a single-step reverse transcription (or transcriptase)-polymerase chain reaction (PCR) (RT-PCR) followed by a semi-nested PCR using an upstream consensus primer and four type-specific primers...

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PMID: 11476326
DOI: 10.1603/0022-2585-38.4.475

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