Article
Genotyping of human papillomavirus in liquid cytology cervical specimens by the PGMY line blot assay and the SPF(10) line probe assay.
Journal of clinical microbiology - 1 Mar 2002
van Doorn Leen-Jan, Quint Wim, Kleter Bernhard, Molijn Anco, Colau Brigitte, Martin Marie-Thérèse, Kravang-In, Torrez-Martinez Norah, Peyton Cheri L, Wheeler Cosette M
Abstract excerpt
A comparison of two PCR-based human papillomavirus (HPV) DNA detection and genotyping systems (PGMY LBA and SPF(10) LiPA) was conducted in two laboratories. Both systems are based on broad-spectrum PCR for the detection of HPV DNA, followed by reverse hybridization with type-specific probes. A total of 400 selected cervical scrape specimens in PreservCyt solution (55% normal cytology, 18% atypical squamous cells...
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