Article
Use of PGMY primers in L1 consensus PCR improves detection of human papillomavirus DNA in genital samples.
Journal of clinical microbiology - 1 Mar 2002
Coutlée François, Gravitt Patti, Kornegay Janet, Hankins Catherine, Richardson Harriet, Lapointe Normand, Voyer Hélène, Franco Eduardo
Abstract excerpt
The novel PGMY L1 consensus primer pair is more sensitive than the MY09 and MY11 primer mix for detection and typing with PCR of human papillomavirus (HPV) DNA in genital specimens. We assessed the diagnostic yield of PGMY primers for the detection and typing of HPV by comparing the results obtained with PGMY09/PGMY11 and MY09/MY11/HMB01 on 299 genital samples. Amplicons generated with PGMY primers were typed...
Topics
- Cervix Uteri
- DNA, Viral
- Female
- Genotype
- HeLa Cells
- Humans
- Papillomaviridae
- Polymerase Chain Reaction
- Vagina
