Article
Use of digoxigenin-labelled oligonucleotide DNA probes for VT2 and VT2 human variant genes to differentiate Vero cytotoxin-producing Escherichia coli strains of serogroup O157.
Journal of clinical microbiology - 1 Jul 1993
Thomas A, Smith H R, Rowe B
Abstract excerpt
Digoxigenin-labelled oligonucleotide DNA probes specific for B-subunit genes of Vero cytotoxin 2 (VT2) and a variant of VT2 (VT2vha) were used to differentiate 116 strains of Escherichia coli serogroup O157 belonging to phage types 1, 2, 4, 8, 14, and 49. Of these strains, 38% had sequences for b...
Topics
- Bacterial Toxins
- Base Sequence
- Coliphages
- Cytotoxins
- DNA Probes
- DNA, Bacterial
- Digoxigenin
- Escherichia coli
- Genes, Bacterial
- Genetic Variation
- Humans
- Molecular Sequence Data
- Polymorphism, Restriction Fragment Length
- Serotyping
- Shiga Toxin 1