Identification of verotoxin type 2 variant B subunit genes in Escherichia coli by the polymerase chain reaction and restriction fragment length polymorphism analysis.

doi:10.1128/jcm.29.7.1339-1343.1991

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Article

Identification of verotoxin type 2 variant B subunit genes in Escherichia coli by the polymerase chain reaction and restriction fragment length polymorphism analysis.

Journal of clinical microbiology - 1 Jul 1991

Tyler S D, Johnson W M, Lior H, Wang G, Rozee K R

Abstract excerpt

A set of synthetic oligonucleotide primers was designed for use in a polymerase chain reaction protocol to specifically detect the B subunit genes in vtx2ha and vtx2hb, which code for the production of the VT2 (Shiga-like toxin II) variant cytotoxins VT2v-a and VT2v-b, respectively. An additional set of primers amplified a fragment common to the B subunits of the VT2 and the VT2 variant genes. Subsequent...

Topics

Animals
Bacterial Toxins
Base Sequence
Cytotoxicity Tests, Immunologic
DNA, Bacterial
Escherichia coli
Genes, Bacterial
Genotype
HeLa Cells
Humans
Molecular Sequence Data

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Neutralization Tests

Polymerase Chain Reaction

Polymorphism, Restriction Fragment Length

Sensitivity and Specificity

Shiga Toxin 1

Vero Cells

Source

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Identifiers

PMID: 1679436
DOI: 10.1128/jcm.29.7.1339-1343.1991
PMCID: PMC270112

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 29 Aug 2026. Prepared record publication-1679436-2026-08-29.1. Manifest publication-graph-cohort-2026-08-29.1.

Source receipt: PubMed via Helena Literature Corpus, retrieved 29 Aug 2026.

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Identification of verotoxin type 2 variant B subunit genes in Escherichia coli by the polymerase chain reaction and restriction fragment length polymorphism analysis.PMID 1679436Read ↗

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