Article
Genetic variability of Yersinia pestis isolates as predicted by PCR-based IS100 genotyping and analysis of structural genes encoding glycerol-3-phosphate dehydrogenase (glpD).
Journal of bacteriology - 1 Feb 2002
Motin Vladimir L, Georgescu Anca M, Elliott Jeffrey M, Hu Ping, Worsham Patricia L, Ott Linda L, Slezak Tomas R, Sokhansanj Bahrad A, Regala Warren M, Brubaker Robert R, Garcia Emilio
Abstract excerpt
A PCR-based genotyping system that detects divergence of IS100 locations within the Yersinia pestis genome was used to characterize a large collection of isolates of different biovars and geographical origins. Using sequences derived from the glycerol-negative biovar orientalis strain CO92, a set of 27 locus-specific primers was designed to amplify fragments between the end of IS100 and its neighboring gene....
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