Article
Cryopreservation of Digitalis obscura selected genotypes by encapsulation-dehydration.
Planta medica - 1 Dec 2001
Sales E, Nebauer S G, Arrillaga I, Segura J
Abstract excerpt
Shoot-tips from several genotypes of the cardenolide-producing perennial shrub Digitalis obscura L. were successfully cryopreserved using the encapsulation-dehydration technique. Precultivation on MS medium containing 0.5 M sucrose, followed by 2.5 h dehydration (final weight 30 %) induced shoot regrowth in 42 % of cryopreserved shoot-tips. Cold-hardening of the in vitro cultures before sucrose treatment...
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