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Detection of Circovirus Infection in Pigeons by in Situ Hybridization Using Cloned DNA Probes

1 Nov 2001

Abstract excerpt

Degenerate primers were designed based on known sequence information for the circoviruses psittacine beak and feather disease virus and porcine circovirus and applied by polymerase chain reaction (PCR) to known virus-infected bursa of Fabricius (BF) from a pigeon. A 548-bp DNA fragment was amplified and shown to be specific to a novel circovirus, named pigeon circovirus (PiCV), and was used to produce sensitive...

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PMID: 11724137
DOI: 10.1177/104063870101300604

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