Diagnosis of duck circovirus infections by conventional and real-time polymerase chain reaction tests

Abstract excerpt

Development of the first conventional and real-time polymerase chain reaction (PCR) tests for the diagnoses of duck circovirus (DuCV) infections is described. Both tests amplified a 230 bp fragment specific to the DuCV Rep gene. Although both tests had the same detection limit (13×103 target DNA/ml) when the target DNA was diluted in water, the detection limit of the real-time test (13×104 target DNA/ml) was...

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