Article
Development of in situ hybridization assay that differentiates between two genotypes of Porcine circovirus-2 in formalin-fixed, paraffin-embedded tissues.
Journal of veterinary diagnostic investigation : official publication of the American Association of Veterinary Laboratory Diagnosticians, Inc - 1 Mar 2010
Kim Duyeol, Ha Yooncheol, Oh Yeonsu, Han Kiwon, Noh Seung Ho, Kim Cheolwoo, Kim Sung-Hoon, Chae Chanhee
Abstract excerpt
The aim of the current study was to develop a nonradioactive in situ hybridization assay that can differentiate between genotypes 2a and 2b of Porcine circovirus-2 (PCV-2) in formalin-fixed, paraffin-embedded lymph node tissues from pigs with postweaning multisystemic wasting syndrome. Two different digoxigenin-labeled oligonucleotide probes were designed from the PCV-2 open reading frame 2 sequences. The...
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