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Quantification of pigeon circovirus in serum, blood, semen and different tissues of naturally infected pigeons using a real-time polymerase chain reaction

25 Mar 2009

Abstract excerpt

The development of a real-time polymerase chain reaction (PCR) based on SYBR Green chemistry is described for the quantification of pigeon circovirus (PiCV) DNA in various samples. Plasmid containing a fragment of the PiCV genome was used to create a standard curve and to estimate the viral DNA copies in analysed samples. Both primers were designed in highly conserved regions to avoid false negatives, and...

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PMID: 19322713
DOI: 10.1080/03079450902737805

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