Article
Speciation of human microsporidia by polymerase chain reaction single-strand conformation polymorphism.
The American journal of tropical medicine and hygiene - 1 Oct 2001
Fedorko D P, Nelson N A, Didier E S, Bertucci D, Delgado R M, Hruszkewycz A M
Abstract excerpt
We describe the application of single-strand conformation polymorphism (SSCP) analysis to the speciation of human microsporidia after polymerase chain reaction (PCR) amplification with the panmicrosporidian primers PMP1 and PMP2. We compared the DNA extracted and amplified from different genotypes or isolates of Enterocytozoon bieneusi, Encephalitozoon cuniculi, E. hellem, and E. intestinalis plus an isolate of...
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