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Identification of microsporidia in stool specimens by using PCR and restriction endonucleases

1 Jul 1995

Abstract excerpt

We report the development of a PCR-based assay for the detection of microsporidia in clinical specimens. A single primer pair complementary to conserved sequences of the small-subunit rRNA enabled amplification of DNA from the four major microsporidian pathogens of humans: Encephalitozoon cuniculi, Encephalitozoon hellem, Enterocytozoon bieneusi, and Septata intestinalis. The extraction method allowed PCR...

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PMID: 7665639
DOI: 10.1128/jcm.33.7.1739-1741.1995

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