Article
Development of a Polymerase Chain Reaction Method for Diagnosing Babesia gibsoni Infection in Dogs.
1 Jan 2001
Abstract excerpt
A pair of oligonucleotide primers were designed according to the nucleotide sequence of the P18 gene of Babesia gibsoni (B. gibsoni), NRCPD strain, and were used to detect parasite DNA from blood samples of B. gibsoni-infected dogs by polymerase chain reaction (PCR). PCR was specific for B. gibsoni since no amplification was detected with DNA from B. Canis or normal dog leucocytes. PCR was sensitive enough to...
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