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Development and Evaluation of a Seminested PCR for Detection and Differentiation of<i>Babesia gibsoni</i>(Asian Genotype) and<i>B. canis</i>DNA in Canine Blood Samples

1 Sept 2003

Abstract excerpt

Canine babesiosis has recently been recognized as an emerging infectious disease of dogs in North America. We sought to develop a seminested PCR to detect and differentiate Babesia gibsoni (Asian genotype), B. canis subsp. vogeli, B. canis subsp. canis, and B. canis subsp. rossi DNA in canine blood samples. An outer primer pair was designed to amplify an approximately 340-bp fragment of the 18S rRNA genes from B....

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PMID: 12958243
DOI: 10.1128/jcm.41.9.4172-4177.2003

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