Article
Promoter-specific involvement of the FixJ receiver domain in transcriptional activation.
Journal of molecular biology - 28 Sept 2001
Ton-Hoang B, Salhi M, Schumacher J, Da Re S, Kahn D
Abstract excerpt
The "two-component" FixLJ system activates nitrogen fixation genes via nifA and fixK in Sinorhizobium meliloti. Like other response regulators, the FixJ protein can be decomposed into an N-terminal phosphorylatable "receiver" domain FixJN and a C-terminal transcriptional activator domain FixJC. The FixJN receiver domain was known to regulate activity of FixJC negatively at the nifA promoter. Here we show a...
Topics
- Alanine
- Bacterial Proteins
- Base Sequence
- DNA Footprinting
- DNA-Binding Proteins
- DNA-Directed RNA Polymerases
- Dimerization
- Gene Expression Regulation, Bacterial
- Genes, Bacterial
- Molecular Sequence Data
- Mutation