Article
The nir, nor, and nos denitrification genes are dispersed over the Bradyrhizobium japonicum chromosome.
Archives of microbiology - 1 Jul 2001
Mesa S, Göttfert M, Bedmar E J
Abstract excerpt
Cleavage of genomic DNA from Bradyrhizobium japonicum strain 3I1b110 by the restriction enzymes PmeI, PacI, and SwaI has been used together with pulsed-field gel electrophoresis and Southern hybridization to locate the nirK, norCBQD, and nosRZDFYLX denitrification genes on the chromosomal map of B. japonicum strain 110spc4. Mutant strains GRK13, GRC131, and GRZ25 were obtained by insertion of plasmid...
Topics
- Anaerobiosis
- Bradyrhizobium
- Chromosome Mapping
- Chromosomes, Bacterial
- Electrophoresis, Gel, Pulsed-Field
- Genes, Bacterial
- Mutation
- Nitrates
- Nitrites
- Nitrogen
- Physical Chromosome Mapping
- Restriction Mapping
