Article
Arbitrarily primed PCR and sequencing of 16S rDNA for epidemiological typing and species identification of Burkholderia cepacia isolates from Swedish patients with cystic fibrosis reveal genetic heterogeneity.
APMIS : acta pathologica, microbiologica, et immunologica Scandinavica - 1 May 2001
Karpati F, Giedraitis V, Thore M, Lindman R, Monstein H J, Hjelte L, Jonasson J
Abstract excerpt
To investigate whether arbitrarily primed (AP)-PCR and/or 16S rDNA sequencing could be used as rapid methods for epidemiological typing and species identification of clinical Burkholderia isolates from patients with cystic fibrosis (CF), a total of 39 clinical B. cepacia isolates, including 33 isolates from 14 CF patients, were fingerprinted. ERIC-2 primer was used for AP-PCR. The AP-PCR clustering analysis...
Topics
- Adolescent
- Adult
- Base Sequence
- Burkholderia
- Burkholderia Infections
- Burkholderia cepacia
- Cystic Fibrosis
- DNA Primers
- DNA, Ribosomal
- Female
- Genes, Bacterial
- Genotype
- Humans