Article
Arbitrarily primed polymerase chain reaction as a rapid method to differentiate crossed from independent Pseudomonas cepacia infections in cystic fibrosis patients.
Journal of clinical microbiology - 1 Oct 1993
Bingen E H, Weber M, Derelle J, Brahimi N, Lambert-Zechovsky N Y, Vidailhet M, Navarro J, Elion J
Abstract excerpt
We used DNA fingerprinting by the arbitrarily primed polymerase chain reaction (AP-PCR) technique for an epidemiological investigation of 23 Pseudomonas cepacia isolates obtained from 11 cystic fibrosis (CF) patients attending our CF center. This approach was compared with ribotyping, pulsed-field gel electrophoresis (PFGE), and conventional phenotypic typing. AP-PCR and ribotyping were identical in resolving...
Topics
- Base Sequence
- Burkholderia cepacia
- Cystic Fibrosis
- DNA Fingerprinting
- Electrophoresis, Gel, Pulsed-Field
- Genotype
- Humans
- Molecular Sequence Data
- Polymerase Chain Reaction
- Pseudomonas Infections