Article
Pulsed EPR/ENDOR characterization of perturbations of the Cu(A) center ground state by axial methionine ligand mutations.
Journal of the American Chemical Society - 6 Jun 2001
Slutter C E, Gromov I, Epel B, Pecht I, Richards J H, Goldfarb D
Abstract excerpt
The effect of axial ligand mutation on the Cu(A) site in the recombinant water soluble fragment of subunit II of Thermus thermophilus cytochrome c oxidase ba(3) has been investigated. The weak methionine ligand was replaced by glutamate and glutamine which are stronger ligands. Two constructs, M160T0 and M160T9, that differ in the length of the peptide were prepared. M160T0 is the original soluble fragment...
Topics
- Base Sequence
- Copper
- DNA Primers
- Electron Spin Resonance Spectroscopy
- Electron Transport Complex IV
- Ligands
- Methionine
- Mutation
- Thermus thermophilus