Discover Community Create You

Loading publication...

Noodle always counts identifier-free feature events for service operation. With your permission, Google Analytics can also estimate audience and engagement. Search text, publication identifiers, cookies, IP addresses and browser fingerprints are never included in Noodle's first-party event counters.

Privacy details

Article

HLA-DQA1 allele typing by nonisotopic PCR-LIS-SSCP.

Brazilian journal of medical and biological research = Revista brasileira de pesquisas medicas e biologicas - 1 Jul 2001

Abba M C, Gómez M A, Golijow C D

Abstract excerpt

In the present study we used a simple and reliable method for HLA-DQA1 allele typing based on the single-stranded conformation polymorphism (SSCP) properties of DNA molecules obtained by PCR. The technique consists of PCR amplification of a DNA fragment comprising the second exon of the HLA-DQA1 gene, amplicon denaturation using a low ionic strength solution (LIS), and electrophoresis on a small native...

Read the complete abstract on PubMed

Topics

Share this publication in a Topic to start or enrich a Post.

Share to HLA-DQ alpha-Chains
Share to HLA-DQ Antigens
Share to Osmolar Concentration

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Share to Alleles

Share to Electrophoresis, Agar Gel

Share to Genotype

Share to Polymerase Chain Reaction

Share to Polymorphism, Single-Stranded Conformational

Source

Identifiers

PMID: 11449304
DOI: 10.1590/s0100-879x2001000700005

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

Related research

Semantic proximity does not establish scientific evidence.

Research trail→

Click a neighbor to travelStep 1 · 8 closest

HLA-DQA1 allele typing by nonisotopic PCR-LIS-SSCP.PMID 11449304Read ↗

PCR-LIS-SSCP (Low ionic strength single-stranded conformation polymorphism)--a simple method for high-resolution allele typing of HLA-DRB1, -DQB1, and -DPB1.Semantic proximity Read ↗

[Detection and genotyping of human papillomavirus (HPV) using PCR-LIS-SSCP].Semantic proximity Read ↗

Discrimination of human HLA-DRB1 alleles by PCR-SSCP (single-strand conformation polymorphism) method.Semantic proximity Read ↗

A simple and rapid method for HLA-DQA1 genotyping by polymerase chain reaction-single strand conformation polymorphism and restriction enzyme cleavage analysis.Semantic proximity Read ↗

Polymerase chain reaction (PCR)/single-strand conformation polymorphism (SSCP) analysis of the human HLA-DQB regions.Semantic proximity Read ↗

Analysis of complex genetic systems by ARMS-SSCP: application to HLA genotyping.Semantic proximity Read ↗

Non-isotopic detection of single-stranded conformation polymorphisms using ethidium bromide/UV light.Semantic proximity Read ↗

HLA-DQA1 genotyping by polymerase chain reaction-single-strand conformation polymorphism (PCR-SSCP) and restriction endonuclease digestion in Papua New Guinea.Semantic proximity Read ↗

⌁Select a neighboring publication to make it the new centre.