Article
Spontaneous mutations affecting transcriptional regulation by protocatechuate in Acinetobacter.
FEMS microbiology letters - 10 Jul 2001
D'Argenio D A, Segura A, Bünz P V, Ornston L N
Abstract excerpt
Positive selection yields Acinetobacter strains with a spontaneous mutation blocking catabolism of protocatechuate. For this study, the growth temperature during selection was lowered to 22 degrees C: growth at 37 degrees C was found to mask the role of the protocatechuate-responsive transcriptional regulator PcaU. The resulting mutants included those with amino acid substitutions useful for understanding PcaU...
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