Article
[Differences in the spatial structure of an envelope protein from tobacco mosaic virus and its mutant, detected by tritium planigraphy].
Molekuliarnaia biologiia - 1 Jan 2000
Lukashina E V, Badun G A, Fedoseev V M, Fedorova N V, Ksenofontov A L, Baratova L A, Dobrov E N
Abstract excerpt
Mutant ts21-66 of the tobacco mosaic virus (TMV) differs from the wild-type TMV-U1 by two mutations (Ile-21-->Thr and Asp-66-->Gly) in the coat protein (CP) gene and in symptoms produced in infected N' plants. The CP structure in TMV-U1 and ts21-66 virions was probed by tritium planigraphy. Compared with the wild-type CP, labeling of the N-terminal region of mutant CP was half as high and suggested its greater...
Topics
- Amino Acid Sequence
- Chromatography, High Pressure Liquid
- Models, Molecular
- Molecular Sequence Data
- Mutation
- Protein Conformation
- Surface Plasmon Resonance
- Tobacco Mosaic Virus
- Viral Envelope Proteins