Article
Closely related Prochlorococcus genotypes show remarkably different depth distributions in two oceanic regions as revealed by in situ hybridization using 16S rRNA-targeted oligonucleotides.
Microbiology (Reading, England) - 1 Jul 2001
West Nyree J, Schönhuber Wilhelm A, Fuller Nicholas J, Amann Rudolf I, Rippka Rosmarie, Post Anton F, Scanlan David J
Abstract excerpt
An in situ hybridization method was applied to the identification of marine cyanobacteria assignable to the genus Prochlorococcus using horseradish-peroxidase-labelled 16S rRNA-targeted oligonucleotide probes in combination with tyramide signal amplification (TSA). With this method very bright signals were obtained, in contrast to hybridizations with oligonucleotides monolabelled with fluorochromes, which failed...
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